Coding

Part:BBa_K2789020

Designed by: Tianyi Chang   Group: iGEM18_WHU-China   (2018-10-06)
Revision as of 10:17, 6 October 2018 by Registry (Talk | contribs)

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Polyphosphokinase that can catalyze the formation of PolyP

This part originally came from 17_Manchester team. But we wanted to get this part and build a more complicated pathway, we found that there is no coding sequence of only PPK without any other protein. Thus we made this part and did an experiment to verify the function of it.It worked well and we found that this kind of PPK can be PCR out of E.coli.BL21. This protein actually can catalyze the formation of heterochromatic granules (Poly P) thus use the natural phosphorus transport system to accumulate the phosphorus in the bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 53
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 732


[edit]
Categories
//cds/enzyme
Parameters
chassisE.coli