Composite

Part:BBa_K2789001

Designed by: Du liaoqi   Group: iGEM18_WHU-China   (2018-10-05)
Revision as of 07:19, 17 October 2018 by Talent b (Talk | contribs)


lac operator+rbs+Ccas+terminator+Ccar+lac operator+rbs+terminator

DNA element : 1.Ccar and Ccas : They could form heterodimer as transcription factor to activate the Cpcg (light control promotor) under green light. And the heterodimer is unstable under blue light.

2.Lac operator : They could block the expression of downstream genes and regain the promotor ability after inducted by IPTG

3.Cpcg : The promotor could be activated by heterodimer made up of Ccar and Ccas

4.TetR : TetR could bind to the tetracydine operator tetO to inhibit transcription .

5.TetO : TetO could be inhibited by tetR

a

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1393
    Illegal BamHI site found at 2973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 798
    Illegal AgeI site found at 1803
    Illegal AgeI site found at 1998
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None