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RNA

Part:BBa_K115004:Design

Designed by: Bastiaan van den Berg   Group: iGEM08_TUDelft   (2008-07-16)
Revision as of 08:18, 16 July 2008 by Registry (Talk | contribs)

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A temperature sensitive 5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The secondary structure is important to the function of these regions, but part of the wt secondary structure is destroyed by the scar. We've tried to alter the sequence so the predicted structure (through mfold and those kind of servers) is sort of conserved, but temperature sensitivity still has to be tested. If it doesn't work, possible solution might be the addition of a larger conserved part of the wt, which implies a small part of wt protein sequence as well.


Source

The sequence is based on the paper "RNA Quadruplex-Based Modulation of Gene Expression" by Markus Wieland and Jorg S. Hartig.

References