Device

Part:BBa_K2556051

Designed by: Yankang Wu   Group: iGEM18_ZJUT-China   (2018-10-05)
Revision as of 22:09, 16 October 2018 by Wuu (Talk | contribs)


f1-AraC-Pbad-lysis-f2

We put AraC-Ara Promoter in front of the lysin gene from phage, and then we use CRISPR/Cas9 technology to turn the construction of AraC-Ara Promoter-Lysis into the genome of E.coli MG1655 wild strain. This part consists of fragments from the E.coli MG1655 genome, AraC-AraPromoter and lysin genes from phages.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2200
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2826
    Illegal NgoMIV site found at 3636
    Illegal AgeI site found at 2035
    Illegal AgeI site found at 4001
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2017


[edit]
Categories
Parameters
None