Composite

Part:BBa_K2812007:Design

Designed by: Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls   Group: iGEM18_TU-Eindhoven   (2018-10-08)
Revision as of 07:59, 8 October 2018 by Registry (Talk | contribs)

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Coding sequence for Pyocin S5 with HlyA and His6-tag regulated by pBAD-ara promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 208
    Illegal BglII site found at 496
    Illegal BglII site found at 2181
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1271


Design Notes

Optimized for E. coli.


Source

...

References