Composite

Part:BBa_K2558214

Designed by: Tsinghua 2018   Group: iGEM18_Tsinghua   (2018-10-05)
Revision as of 11:49, 5 October 2018 by Registry (Talk | contribs)

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Neon lux positive feedback device with luxpR-HS promotor

This construct is the NEON positive feedback device, containing constantly expressed luxR, lux pR-HS (K2258001) controlled luxI, and lux pR-HS (K2258001) controlled GFP. Without AHL stimulation, the lux pR-HS promotor is repressed by the dCas9-gRNA expressed by Safety Catch system. Therefore, the luxI and GFP cannot be expressed. Upon the stimulation of AHL, the CRISPRi system in Safety Catch is inhibited, thus the lux pR-HS promotor is exposed, and then luxR-AHL complex now can bind and activate lux pR-HS promotor. Thereofre, luxI and GFP can now be expressed. Besides, this system will keep producing more luxI-AHL, which will form a positive feedback and increase the expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2026
    Illegal NheI site found at 2049
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 801
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 950
    Illegal SapI.rc site found at 1129


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