Composite

Part:BBa_K2812003:Design

Designed by: Guido Oerlemans, Maxime van den Oetelaar and Mariska Brüls   Group: iGEM18_TU-Eindhoven   (2018-10-03)
Revision as of 21:54, 16 October 2018 by Maximeoet (Talk | contribs)


Coding sequence for trunctated Lysostaphin regulated by T7-promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The initial starting sequence for truncated lysostaphin was taken from BBa_K748002. This amino acid sequence was used to generate a codon optimized sequence for expression in E. coli.

Source

The designed sequence was synthesized de novo by IDT.