Part:BBa_K2556051
f1-AraC-Pbad-lysis-f2
With the wide application of recombinant DNA technology, biotechnology has undergone a qualitative leap. Many of the genetic engineering products are intracellular substances, so the cell wall must be broken down so that the product can be released before it can be further extracted, so cell fragmentation is a key step in extracting intracellular products, and the method of fragmentation is appropriate or not. Directly affect the output, quality and production cost of the extracted products. In recent years, the methods of cell fragmentation are as follows: high pressure homogenization, high speed bead milling, ultrasonic crushing, enzyme dissolution and chemical permeation. These methods may have harmful products, high cost or low efficiency. For this reason, we plan to construct a strain of engineering bacteria that can be cracked by adding arabinose. We put AraC-Ara Promoter in front of the lytic gene from phage, and then we use CRISPR/Cas9 technology to turn the construction of AraC-Ara Promoter-Lysis into the genome of E.coli MG1655 wild strain. This part consists of fragments from the E.coli MG1655 genome, AraC-AraPromoter and lytic genes from phages.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2200
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2826
Illegal NgoMIV site found at 3636
Illegal AgeI site found at 2035
Illegal AgeI site found at 4001 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2017
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