Composite
Part:BBa_K2680262
Designed by: Ethan M Jones Group: iGEM18_William_and_Mary (2018-10-06)
3G KanR
3G KanR; Kanamycin resistance gene flanked by prefix sticky end C and suffix sticky end D in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].
"Kanamycin resistance with two amber stop codons. The part was designed for the use in a positive selction plasmid to attain aa specific tRNA/synthetase, able to incorporate non canonical amino acids. The Kanamycin resistence is desined with two amber codons, resulting in a lacking resistence if an endogeneous amino acid ist incorporated."1
References
1
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 47
Illegal BsaI.rc site found at 879
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Categories
Parameters
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