Coding

Part:BBa_K2729010

Designed by: Hajime Fujita   Group: iGEM18_Tokyo_Tech   (2018-09-30)
Revision as of 18:23, 16 October 2018 by Crane (Talk | contribs)


DENV1 ancC-prM-E (Dengue Virus Serotype I)

Usage and Biology

Flavivirus Capsid (C), prM (precursor Membrane) and Envelope (E) are structural proteins responsible for gathering the viral RNA into a nucleocapsid that forms the core of a mature virus particle.

Characterization

The prM protein consists of an N-terminal pr domain followed by the M protein separated by a furin cleavage site.(15) The pr part of the protein consists mainly of β-strands, whereas the M portion consists of a linear structure followed by a mainly α-helical stem region and two transmembrane helices.

Figure 1: Result of the Electrophoresis


As shown in Figure 1, the band of DENV4 prME come around 2,000 bp.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1831
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1831
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1245
    Illegal BglII site found at 1856
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1831
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1831
    Illegal AgeI site found at 1450
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 217
    Illegal BsaI.rc site found at 367

Reference

- Membranotropic regions of the dengue virus prM protein

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