Coding

Part:BBa_K2570019:Design

Designed by: Luying He   Group: iGEM18_FJNU-China   (2018-10-03)
Revision as of 04:33, 4 October 2018 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


tetR+GFP+mazF+PLtetO1+RFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2590
    Illegal AgeI site found at 2702
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have added an enzyme cutting site in the design to facilitate BIOBRICK.


Source

We ordered the part DNA from a synthesis company and had codon optimization of the sequence.

References