Composite

Part:BBa_K2558206

Designed by: Tsinghua 2018   Group: iGEM18_Tsinghua   (2018-10-05)
Revision as of 11:37, 5 October 2018 by Registry (Talk | contribs)

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CRISPRi device with Ptac expressed gRNA and Anderson strong (J23100)- expressed lacI

This construct is the CRISPRi device, which is composed of dCas9, Ptac controlled gRNA targeting PHIF repressible promotor (BBa_K1725000) and constitutively (BBa_J23100, Anderson strong promotor) expressed lacI protein. We designed this device along with K2558207 and K2558208, which are the same except for the strength of the constitutive lacI expression. We aim to test the repression efficiency of our CRISPRi system with the help of PHIF repressible promotor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 310
    Illegal NheI site found at 333
    Illegal NheI site found at 1466
    Illegal NheI site found at 4626
    Illegal NheI site found at 4649
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5830
    Illegal BamHI site found at 3745
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None