Coding

Part:BBa_K2680542:Design

Designed by: Adam Oliver   Group: iGEM18_William_and_Mary   (2018-10-16)
Revision as of 14:47, 16 October 2018 by Registry (Talk | contribs)

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KanR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence sent to William and Mary iGEM from Murray Lab at California Institute of Technology. Used PCR to add William and Mary Pad overhangs, which were used to clone the part into William and Mary Pad backbone. Forbidden restriction sites were checked for and removed if necessary.



Source

Sequence sent to William and Mary iGEM from Murray Lab at California Institute of Technology.


References