Coding
Part:BBa_K2729008
Designed by: Hajime Fujita Group: iGEM18_Tokyo_Tech (2018-09-30)
ZsYellow-FMDV 2A
YFP is a genetic variant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm. FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 43
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 289
Illegal NgoMIV site found at 367
Illegal NgoMIV site found at 820
Illegal AgeI site found at 632 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |