Coding
Part:BBa_K2729007
Designed by: Hajime Fujita Group: iGEM18_Tokyo_Tech (2018-09-30)
DsRed-Express-FMDV 2A
DsRed, a brilliantly red fluorescent protein, was recently cloned from Discosoma coral by homology to the green fluorescent protein (GFP) from the jellyfish Aequorea. The DsRed-Monomer sequence contains 45 amino acid substitutions but maintains a spectral profile virtually identical to other DsRed fluorescent proteins. FMDV 2A is used widely for this purpose since multiple proteins can be coexpressed by linking open reading frames (ORFs) to form a single cistron.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 43
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 808
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |