Part:BBa_K2549062

CMV-CD8α-EGFP-PDGFRβ

This part is a surface-expressed EGFP which can be served as antigen to the SynNotch receptor. CMV promotor is a constitutive promotor. CD8α signal peptide is used to guide synthesized fusion protein to pass the translocon^[1] into the endoplasmic reticulum^[2], and the fusion protein will be later sugar modified in Golgi^[3], presented on the plasma membrane and located to the outside of the cell. Transmembrane region of PDGFRβ embeds surCD19 on the membrane. This part is copied from our viral vector which can be expressed in mammalian cells. It is served as one of our antigen to SynNotch receptors.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 531
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 540
1000
COMPATIBLE WITH RFC[1000]

Characterization

We used fluorescence microscopy to capture the EGFP which be successfully expressed on the surface of 293T cell.

Fig.1 EGFP is detected to be expressed on the plasma membrane of 293T cell.

References

[1] ttps://en.wikipedia.org/wiki/Translocon

[2] ttps://en.wikipedia.org/wiki/Endoplasmic_reticulum

[3] ttps://en.wikipedia.org/wiki/Golgi_apparatus

[1]

[2]

[3]