Regulatory

Part:BBa_K2610021

Designed by: Charlotte de Ceuninck van Capelle, Daphne van den Homberg   Group: iGEM18_Leiden   (2018-10-09)
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pRpoE

The gene rpoE encodes the sigma factor E, a protein in Escherichia coli and other species of bacteria. RpoE appears to be necessary for the exocytoplasmic stress response. E. coli mutants without rpoE are not able to grow at temperatures above 42 degrees Celsius.

Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.

T--Leiden--heatmap.png

Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the RpoE promoter is activated by, amongst others, chloramphenicol, ethanol and nalidixic acid.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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