Part:BBa_K2637021

AD-KaiC, Gal4 activation domain fused with KaiC

This fusion protein is made up of Gal4 activation domain and KaiC and fuctions as a part of yeast two-hybrid system. When KaiC binds to another part of the system-SasA, CikA, or KaiB, AD simultaneously binds to upstream genes and initiates the transcription of downstream genes.To see more about the sequence or other things, visit BBa_K2637007 and BBa_K2637003.

Notes: You can select this part and part BBa_K2637022 to make up a yeast two-hybrid system and finally constructe an oscillator. And you can add a specific promoter to this part to regulate the expression of your reporter gene. If you want a integrated yeast two-hybrid system, you can select other combination. You can find that on part page in our wiki.

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Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 670
  Illegal BglII site found at 1177
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]