Part:BBa_K2582001

RNA Aptamer Probe for N9 (545-566)

This biobrick is a miniSpinach-based RNA Aptamer Probe (RAP). It targets the influenza Neuraminidase subtype 9 (Gene accession number: CY235364.1) by nucleotide 545-566.

Illustration of the design

Usage and Biology

The full miniSpinach allows the docking of fluorogen 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) and increases its quantum yields by hundreds of folds. By truncating the critical P1 arm and attaching it to a 22-bp RNA-specific probe, the RNA Aptamer Probe can bind to a RNA sequence and enhances the fluorecence level of the docking DFHBI subsequently. In our work, this probe is demonstrated to be functional in vitro, targeting influenza RNA fragments. It also has the potential for live-cell reporting of mRNA level (Ong. et. al. 2017.).

The probe can be used for on-site, rapid and sequence-specific influenza detection. Its target neuraminidase gene encodes for one of the influenza viral protein that determines the subtype of the virus, which is a important factor for epidemic monitoring and some treatment schemes.

Characterization

Specificity

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]