Coding

Part:BBa_K2596005:Design

Designed by: Matthew Mullin   Group: iGEM18_Stony_Brook   (2018-10-10)
Revision as of 04:49, 16 October 2018 by Kledalla (Talk | contribs) (→‎Design Notes)


cscB (sucrose permease) from E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 481
    Illegal NgoMIV site found at 604
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We codon optimized cscB for our cyanobacterial chassis. However, after optimization, there was an illegal PstI cut site. We were able to remove the cut site through a silent point mutation. The codon that we replaced it with was fortunately equally as abundant as the prior codon.

Source

E. coli

References