Coding

Part:BBa_K2764000

Designed by: CHU Ora-In   Group: iGEM18_Hong_Kong_HKUST   (2018-10-03)
Revision as of 03:09, 16 October 2018 by Yhlauah (Talk | contribs)


Alkane channel protein

The alkL protein is an alkane channel protein that comes from Pseudomonas Oleovorans. Its function is mainly to import medium to long chain alkanes into the cell. In Gram-negative cells, such as Escherichia coli and Shewanella oneidensis, import of medium to long chain alkanes is inhibited by the cell's lippopolysaccharide layer. The alkL protein's extracellular domain penetrates this layer and binds to alkane molecules with high affinity. Once the alkane has been bound, it is transported through the hydrophobic core of the alkL protein and deposited into the periplasm of the cell, where it can then diffuse into the cytoplasm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 651
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 303
    Illegal NgoMIV site found at 516
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None