DNA

Part:BBa_K2614000:Design

Designed by: Nicole Banholzer, Rick Seifert   Group: iGEM18_JMU_Wuerzburg   (2018-10-09)
Revision as of 22:00, 15 October 2018 by Mudskipper (Talk | contribs) (→‎Source)


template as positive control for a qPCR that detects Plasmodium


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We amplifiy the template by a PCR and digest it with EcoRI-HF an PstI-HF. Subsequently we digest the pSB1C3 plasmid with EcoRI-HF and PstI-HF and ligate the digested Plasmid with our digested template.


Source

As template we used a short, synthetic, non toxic, non pathogene oligonucleotide.

References