RBS

Part:BBa_K2675015

Designed by: Esteban Lebrun   Group: iGEM18_Evry_Paris-Saclay   (2018-10-05)
Revision as of 09:41, 12 October 2018 by ELebrun (Talk | contribs)


Synthetic RBS designed for sfGFP

This part is a synthetic RBS designed for sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) using the Salis Lab RBS calculator v2.0 [1, 2].

Usage and Biology

This RBS was used to drive the expression of sfGFP (BBa_K2675005) and sfGFP-LVAtag (BBa_K2675006) under the control of the pAimX short promoter (BBa_K2675021) or its derivative (BBa_K2675022) in the composite parts BBa_K2675051, BBa_K2675052, BBa_K2675058, BBa_K2675059, BBa_K2675061 & BBa_K2675062. 

Using the  Salis Lab RBS calculator v2.0 [1, 2], the predicted features of this RBS are:
Translation Initiation Rate (au)	:	202865,54
dG_total (kcal/mol)	:	-11,34
dG_mRNA_rRNA (kcal/mol)	:	-20,08
dG_spacing (kcal/mol)	:	0
dG_standby (kcal/mol)	:	0
dG_start (kcal/mol)	:	-2,76
dG_mRNA (kcal/mol)	:	-12,28
Accuracy (warnings)	:	OK

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None