Part:BBa_K2878000

T7 promoter sequence

T7 promoter oligo, we added this ologo sequence to the 5' end of oligo DNA template sequence for shRNA transcription. So that shRNAs can be generated in large scale through in vitro transcription system.

Usage

We added T7 promoter to the 5-prime end of each shRNA template sequence for in vitro transcription of the shRNAs,

Biology

T7 promoter is originally obtained from bacteriophage T7, it is specific for bacteriophage T7 RNA polymerase. Bacteriophage T7 (or the T7 phage) is a bacteriophage, a virus that infects susceptible bacterial cells, that is composed of DNA and infects most strains of E. coli. The T7 promoter is widely used for in vitro transcription of a DNA template. The T7 promoter is also commonly used in E. coli expression studies.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]