Part:BBa_K2559003

Ecoli promoter of sgRNA(PrplJ)

The rpij promoter is a strong endogenous promoter in Escherichia coli K 12;we use it to start the expression of single guide RNA

Usage and Biology

We have obtained this promoter from a database, PromEC ( http://margalit.huji.ac.il/promec/index.html) .PromEC is an updated compilation of E. coli mRNA promoter sequences. It includes documentation on the location of experimentally identified mRNA transcriptional start sites on the E. coli chromosome, as well as the actual sequences in the promoter region. The database is currently updated as of July 2000 and includes 471 entries. We have built up a model to measure the expression strength of these promoters and tested the prplJ, pdapA, and pcaiF expression strength by comparing the fluorescent intensity.You can use PromEC and our model to obtain more useful promoter information. The eGFP expression in E.coli(DH5 alpha) and was driven by prplj/pdapa/pcaif promoter.

Figure1 The result of the fluorescent intensity measurement

Figure2 Fluorescent intensity of eGFP driven by by rplj/dapa/caif promoter.

Due to model predition and experiment test result,we use prplJ as the promoter of sgRNA The part is facilitate the in-depth research for other teams!

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]