Composite

Part:BBa_K2680124

Designed by: Ethan M Jones   Group: iGEM18_William_and_Mary   (2018-10-06)
Revision as of 22:07, 14 October 2018 by Jaurban (Talk | contribs)


3G pTlpAr

pTlpAr flanked by prefix sticky end A and suffix sticky end B in a 3G-compatible backbone. For more information on 3G hybrid DNA assembly and 3G parts, see the [http://2018.igem.org/Team:William_and_Mary/Part_Collection William and Mary 3G Parts Page].

pTlpAr is the reverse complement of the heat-sensitive promoter pTlpA. Transcribes 200-fold weaker than pTlpA.1 Flanked by prefix sticky end A and suffix sticky end B.

References

1Piraner, D. I., Abedi, M. H., Moser, B. A., Lee-gosselin, A., & Shapiro, M. G. (2017). Tunable thermal bioswitches for in vivo control of microbial therapeutics. Nature Chemical Biology, 13(1), 75-80. doi:http://dx.doi.org/10.1038/nchembio.2233


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 41
    Illegal BsaI.rc site found at 109


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Categories
Parameters
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