RNA

Part:BBa_K2556061

Designed by: Yankang Wu   Group: iGEM18_ZJUT-China   (2018-10-04)
Revision as of 03:30, 5 October 2018 by Registry (Talk | contribs)

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sgRNA-cm

Single-guide RNA (sgRNA) is an artificial RNA which is designed to bind a certain DNA sequence. It contains the 20-bp complementary region (N20) with the requisite NGG PAM matching genomic loci of interest and the sequence of tracrRNA. It will guide the nuclease Cas protein 9(Cas9) to the target. The N20 of sgRNA-cm is complementary to the 20 bp fragment of the chloramphenicol gene. When cas9 protein is present, sgRNA-cm will guide cas9 to cleave the chloramphenicol resistance gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None