Device

Part:BBa_K2556011

Designed by: Yankang Wu   Group: iGEM18_ZJUT-China   (2018-10-04)
Revision as of 04:51, 5 October 2018 by Wuu (Talk | contribs)

Light-involved system control the expression of Cas9


YF1 is a fusion protein of a LOV protein domain and histidine kinase. In the dark YF1 phosphorylates FixJ response regulator and phospholylated FixJ response regulator activates Pfixk2 promoter. In blue light(480 nm) YF1 don’t phosphorylate FixJ response regulator, so Pfixk2 promoter isn’t activated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 3356
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 5635
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218


[edit]
Categories
Parameters
None