Device

Part:BBa_K2841500

Designed by: Jack Lawrence, Rhys Evans, Jonny Whiteside   Group: iGEM18_Warwick   (2018-10-09)
Revision as of 13:52, 14 October 2018 by Psychosahedron (Talk | contribs)


Legionella Sensing LacI Repressing Alpha Toxicity Experimental Device

GFP is expressed with the gRNA to test toxicity


IPTG Induction
IPTG OD600
IPTG Induction Fluorescence




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1077
    Illegal NgoMIV site found at 1106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 856


[edit]
Categories
Parameters
None