Coding

Part:BBa_K2660008

Designed by: Danielle Biscaro Pedrolli,Victor Nunes de Jesus   Group: iGEM18_Unesp_Brazil   (2018-10-08)
Revision as of 13:12, 14 October 2018 by VictorNJ (Talk | contribs)


nMag - Light-activated protein

This photoswitch made from a blue light photoreceptor VVD derived from the filamentous fungus Neurospora crassa. The VVD is currently one of the smallest photoreceptors among those tested as optogenetic tools. The negative Magnet (nMag)<b> when irradiated by a blue light change through electrostatic interactions.


This part can be used with pMag (BBa_K2660009) to join different domains of a protein of interest when irradiated by blue light


<b>References
Kawano, F. et al. Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins. Nat. Commun. 6:6256 doi: 10.1038/ncomms7256 (2015)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 87
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/bsubtilis
//collections/probiotics/biocontainment
Parameters
None