Regulatory

Part:BBa_K2610025

Designed by: Charlotte de Ceuninck van Capelle   Group: iGEM18_Leiden   (2018-10-09)
Revision as of 12:56, 14 October 2018 by Chardeceuninck (Talk | contribs)


pSpy

Spy, an ATP-independent chaperone, is highly upregulated in the presence of protein unfolding agent ethanol. It decreases protein degradation.

Usage and Biology

iGEM Leiden 2018 has created a screening system for the detection of new antibiotics. We have tested several stress-activated promoters against a repertoire of known antibiotics (Figure 1) using flow cytometry. We fused promoters to green fluorescent protein and stressed transformed DH5a cells for several hours.

T--Leiden--heatmap.png

Figure 1. Heatmap with stress-activated promoters

As can be observed in Figure 1 the SpY promoter is activated by nalidixic acid, ethanol and chloramphenicol. This is indicative of a similar mechanism of actions for these compounds.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None