Part:BBa_K2637043
Gal1 promoter+EYFP+ADH1 terminator+Gal2 promoter+NanoLuc+CYC1 terminator
We constructed this pRS416 plasmid which contained two cassettes consisting of Gal1 promoter with EYFP and ADH1T terminator and Gal2 promoter with Fluc and CYC1 terminator by harnessing the principles of yeast homologous recombination. The plasmid incorporating these two cassettes which played a prominent role in our measurement process would be a segment of our parallel experiments aimed to minimize the false positive phenomenon of the yeast two-hybrid system. In detail, we built up the core oscillation system in the Saccharomyces cerevisiae, which originally can’t be directly observed or detected, but this oscillation information could be exhibited by the visible data images by utilizing the yeast two-hybrid system. In consideration of the yeast two-hybrid system which is prone to having false positive, we constructed this plasmid to minimize this phenomenon, allowing our conclusions to be more reliable. Only the data images embodied that the genes of EYFP and NanoLuc had been synchronously oscillating expressed can we ensure that our oscillation system definitely operated.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2913
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1125
Illegal SapI site found at 2121
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