Composite

Part:BBa_K2637042

Designed by: Jinyu Yang   Group: iGEM18_Tianjin   (2018-10-05)
Revision as of 14:26, 5 October 2018 by Registry (Talk | contribs)

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Gal1 promoter+mCherry+ADH1 terminator+Gal2 promoter+NanoLuc+CYC1 terminator

We constructed this pRS416 plasmid which contained two cassettes consisting of Gal1 promoter with mCherry and ADH1T terminator and Gal2 promoter with NanoLuc and CYC1 terminator by harnessing the principles of yeast homologous recombination. And only if the reporter genes function normally can we ensure that the system we reconstructed in the Saccharomyces cerevisiae succeeds.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2890
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2098


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Categories
Parameters
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