Composite

Part:BBa_K2637041

Designed by: Jinyu Yang   Group: iGEM18_Tianjin   (2018-10-05)
Revision as of 13:58, 5 October 2018 by Registry (Talk | contribs)

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Gal2 promoter+NanoLuc+ADH1 terminator

We constructed this pRS416 plasmid carrying the genes of NanoLuc with the Gal2 promoter and ADH1T terminator by harnessing the principles of yeast homologous recombination. And only if the reporter genes function normally can we ensure that the system we reconstructed in the Saccharomyces cerevisiae succeeds.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 529


[edit]
Categories
//cds
//cds/reporter
//chassis/eukaryote/yeast
//function/reporter/light
Parameters
None