Coding

Part:BBa_K1469009

Designed by: Philip Hartz   Group: iGEM14_Saarland   (2014-10-02)
Revision as of 04:02, 14 October 2018 by Lihuimin (Talk | contribs) (→‎Improvement)


gcaD

UDP-N-acetylglucosamine pyrophosphorylase of Bacillus megaterium. The enzyme is bifunctional and catalyses the conversion of glucosamine 1-phosphate to N-acetyl glucosamine 1-phosphate as well as the conversion of N-acetyl glucosamine 1-phosphate to UDP-N-acetyl glucosamine, a key precursor molecule for hyaluronic acid production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 220


=====Improvement=====

2018 SSTi-SZGD erified that glmU increases HA production. glmU encodes a UDP-N-acetylglucosamine pyrophosphorylase.  it has 495 amino aicd   (also know as : GcaD in megaterium)
168E-glmU produces precipitates formed by the reaction of HA with CTAB, OD400:2.313. HA’s concentration is 447.2mg/L.

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Categories
Parameters
None