Composite
NG4

Part:BBa_K2623024

Designed by: Yunyun Hu   Group: iGEM18_XMU-China   (2018-10-02)
Revision as of 14:45, 13 October 2018 by NickCai (Talk | contribs)


A non-GFP form of the BBa_K2623023

This part was the non-GFP form of BBa_K2623023. It's also another form of BBa_K2623022 in which SpyTag was inserted to OmpA protein's C-termini, designed to target our archaeal ribosomal protein protein L7Ae and then siRNA into outer-membrane vesicles (OMVs). Porin outer-membrane protein A, OmpA, is a kind of highly expressed transmembrane porin protein of E. coli and is therefore abundant in OMVs. We inserted SpyTag to its C-termini and SpyCather to L7Ae (BBa_K2623026) in order to anchore L7Ae to outer membrane through the bio-conjugation of the SpyTag/SpyCatcher system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 65
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None