Composite

Part:BBa_K2865006

Designed by: Hu Miao   Group: iGEM18_SMMU-China   (2018-10-02)
Revision as of 16:26, 13 October 2018 by WendyCui (Talk | contribs)


CMV promoter-eGFP-poly(A)

This part contains CMV promoter, eGFP reporter in RFC25 format(BBa_K2865005), and SV40 polyA terminator (BBa_K2865004). This part is constructed as a control with our part BNP promoter-eGFP-poly(A) (BBa_K2865008).

 

Fig.1 the construction of part

Experiment

Fluorescence microscope

We transfected H9C2 cell with target plasmids, BBa_K2865006 and BBa_K2865005, via liposome transfection. After 48 hours’ expression, we detected the cells using fluorescence microscope. And we have taken clearly photos. Wo all know that the CMV promoter is widely and commonly used in plasmids or viruses designed for gene expression in mammalian cells since it is a very strong promoter and drives constitutive expression of genes under its control. And we have to admit that the transfection efficiency is low.

 

Figure 2 Expression of CMV-eGFP-poly(A) was taken under fluorescent microscope and light microscope.

 

CMV promoter-eGFP-poly(A) served as the positive control. It contains the CMV promoter and eGFP reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1430
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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