Coding
Part:BBa_K2598011:Design
Designed by: Matthew Gao Group: iGEM18_UCAS-China (2018-09-22)
T7 RNAP sigma fragment CGG
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 578
Illegal NgoMIV site found at 1193
Illegal NgoMIV site found at 1202 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
It's obtained by Segallshapiro T H et al
References
Segall-Shapiro, T. H., Meyer, A. J., Ellington, A. D., Sontag, E. D. & Voigt, C. A. A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol 10, 742, (2014)