Conjugation

Part:BBa_I714031

Designed by: Mingzhi Qu   Group: iGEM07_Peking   (2007-10-21)
Revision as of 13:48, 10 October 2018 by Shemy (Talk | contribs)


OriT-R (Origin of Transfer for the R751-plasmid nic region)

OriT-R751 (Origin of Transfer for the R751-type conjugative plasmid) is the nic region, where the relaxosome nicks the plasmid and conjugative transfer by R751 plasmid machinery begins via rolling circle replication.

Characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 51
    Illegal NgoMIV site found at 104
    Illegal NgoMIV site found at 114
  • 1000
    COMPATIBLE WITH RFC[1000]


There is a point error(G->A) in No.108bp. Utilizing PCR can realize point mutation, so the key point is the primer design. The forward primer is 5’-cgcctcgtctctcatgccggcggtagccggctgcc-3’, and the reverse primer is 5’-gcgaggcagccggctaccgccggcatgagagacgaggcg-3’.

[edit]
Categories
//DNA/conjugation
//function/conjugation
Parameters
None