Part:BBa_K2560259:Design
mcr gene for Malonyl-CoA Reductase from Sulfolobus tokodaii
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 626
Illegal BglII site found at 819 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part contains the whole coding region from the mcr gene of S. tokodaii (OOOOO) without the startcodon. This region is flanked by overhangs which are Phytobrick- and MoClo-compatible and by two BsaI recognition sites (Weber et al., 2011).
GGTCTCGGATG-coding_region-GCTTTGAGACC
The sequence was codonoptimized for V. natriegens ATCC 14048.
The encoded enzyme catalyzes to conversion of malonyl-CoA into 3-oxopropanoate. By combining this part with BBa_K2560262, it is possible to produce 3-hydroxypropionate from malonyl-CoA.
Source
Source of the part:
Genome: Sulfolobus tokodaii strain 7
Accession number of gene: ST2171
Accession number of encoded protein: BAB67276
mcr was codonoptimized for V. natriegens and then synthetisized and integrated into the vector BBa_K2560002 via BsmBI
References
Strauss, G., Fuchs, G., 1993. Enzymes of a novel autotrophic CO2 fixation pathway in the phototrophic bacterium Chloroflexus aurantiacus, the 3-hydroxypropionate cycle. Eur. J. Biochem. 215, 633–43.
Weber, E., Engler, C., Gruetzner, R., Werner, S., Marillonnet, S., 2011. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS One 6, e16765. https://doi.org/10.1371/journal.pone.0016765