Reporter

Part:BBa_K2607002:Design

Designed by: Valdi Ven Japranata   Group: iGEM18_UI_Indonesia   (2018-09-25)
Revision as of 16:28, 12 October 2018 by Valdiven (Talk | contribs) (→‎Design Notes)


Improved Blue Fluorescent Protein (BFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is assembled as improvement of B0034-Blue Florescent Protein (BFP) (BBa_K592024). Our established part consists of these following features:

  • lacI regulated promoter (BBa_R0010) in the upstream
  • Double terminator (BBa_B0010 and BBa_B0012) in the downstream
  • SalI restriction site between the promoter and ribosome binding site
  • NdeI restriction site between the ribosome binding site and coding sequence
  • Modified blue fluorescence protein coding sequence to eliminate SalI restriction site in the middle without altering amino acid sequence

Source

To be added

References