Coding

Part:BBa_K2547004

Designed by: Luyao Pan   Group: iGEM18_AHUT_China   (2018-10-04)
Revision as of 11:21, 12 October 2018 by YuruChen (Talk | contribs)


Carbonic anhydrase 2 (L203K)

This part is the coding sequence (CDS) of human carbonic anhydrase (CA2), because the carbonic anhydrase (CA2) extracted in human body has the fastest reaction rate at 37 ° C, and loses at 50 ° C. Live, so the DNA molecule was mutated and found that when the amino acid encoded by the 203th codon was mutated from leucine to lysine, the carbonic anhydrase produced had higher thermal stability and was encoded. The addition of a histidine tag (His-Tag) following the sequence (CDS) facilitates the purification of the carbonic anhydrase protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None