Deleted
Sample Discontinued
Experience: None
Not Used
Get This Part
RNA

Part:BBa_K2591021

Designed by: Luo Kevin   Group: iGEM18_SUSTech_Shenzhen   (2018-10-11)
Revision as of 06:26, 11 October 2018 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


A gRNA designed to Wls, an essential gene to secretion of the canonical wnt protein

Single-gRNA is a small RNA which help CRISRR-Cas protein family to target the exogenous sequence, and assist the prokaryotes to defend phages. Nowadays, the artificial gRNA can achieve the modification casually in the gene level and is a excellent way to introduce mutation. In our project, we design this gRNA for wntless WNT ligand secretion mediator (Wls), and it is a functional protein for the secretion of the Wnt3A protein and assist the modification for the mature Wnt3A protein. Therefore, if we use the gRNA for Wls, we can inhibit the donor cell to secret the Wnt3A protein normally.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None