RNA

Part:BBa_K2889000:Experience

Designed by: Rui Zhou   Group: iGEM18_Worldshaper-Wuhan   (2018-10-04)
Revision as of 08:01, 11 October 2018 by Ruizhou (Talk | contribs) (→‎Applications of BBa_K2889000)


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Applications of BBa_K2889000

1.cell migration 1.1 Cell migration is a central process in the development and maintenance of tumor. We cloned the full length of IL7-AS-S2 into PCDNA3.1 and transfected the plasmid to 786-O cells.Through in vitro scratch wound healing assay, overexpression of IL7-AS-S2 promoted cell migration of 786-O cells.Overexpression of IL7-AS-S2 increased the migration of 786-O cells (Fig. 1 and 2). These results suggested that IL7-AS-S2 contain key structural domains.

IL7-AS-AS2.jpg


IL7-AS-S2-cell_migration.jpg


1.2 In order to investigate whether cell migration induced by IL7-AS-S2 depends on the amount of IL7-AS-S2 transfection. We transfected the different concentration of IL7-AS-S2 () into 786-0 cells.(Fig. 3 and 4)


Different_concentration_of_IL7-AS-S2.jpg


Different_concentration_of_IL7-AS-S2-.jpg

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