Coding

Part:BBa_K2788001

Designed by: Mingyue Luo   Group: iGEM18_SZU-China   (2018-10-05)
Revision as of 06:38, 11 October 2018 by M Orz (Talk | contribs) (→‎iGEM2018 SZU-China)


Bbchit_Beauveria bassiana ARSEF 2860

This part is the coding sequence (CDS) of Chitinase from The Beauveria bassiana ARSEF 2860(GenBank Acc.No.txid655819). It can encode chitinase and effectively decomposes chitin. We transferred this gene to enhance the penetration of Metarhizium anisopliae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 581
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 568
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 250
    Illegal NgoMIV site found at 779
  • 1000
    COMPATIBLE WITH RFC[1000]


iGEM2018 SZU-China

In order to make Metarhizium anisopliae penetrate the corpus callosum more efficiently, we constructed an expression vector containing part Bbchit(Fig.1)

Fig.1 Construction of expression vector Bbchit-pBC. PgpdA and TtrpC come from parts of 2016_NYMU-Taipei: BBa_K2040101 and BBa_K2040102, and Bbchit comes from the Beauveria bassiana ARSEF 2860. The PgpdA-Bbchit-TtrpC part is connected to the pBC plasmid through the BioBrick site.
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Categories
Parameters
None