Composite
Part:BBa_K2539400
Designed by: Justin Wu Group: iGEM18_TAS_Taipei (2018-09-03)
PalcA-Regulated ALDH2*1 Expression Construct
A regulated ALDH2*1 expressing construct that employs the ethanol and alcR induced promoter (BBa_K2092002) and a strong RBS (BBa_B0034) to regulate expression of ALDH2*1, a mitochondrial enzyme responsible for the oxidation of acetaldehyde into acetate. The sequence is then terminated with a double terminator (BBa_B0015) to ensure the end of transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 274
Illegal BglII site found at 2116 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1174
Illegal NgoMIV site found at 1317
Illegal NgoMIV site found at 1830 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1236
Illegal SapI.rc site found at 1733
[edit]
Categories
Parameters
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