Composite

Part:BBa_K2539300

Designed by: Justin Wu   Group: iGEM18_TAS_Taipei   (2018-09-03)
Revision as of 09:36, 8 October 2018 by Juster (Talk | contribs) (→‎Characterization)


AlcR Expression Construct

This construct was built to overexpress alcR, a regulatory protein necessary for the activation of palcA (BBa_K2092002). We acquired all parts from the iGEM distribution kit: a strong promoter and strong RBS combination (BBa_K880005) to maximize protein production, strong RBS (BBa_B0034), alcR (BBa_K2092001), and a double terminator (BBa_B0015) to end transcription. PCR checks and sequencing results from Tri-I Biotech confirmed that our final alcR construct is correct.


Characterization

AlcR is a transcription factor that, along with substances like ethanol, activates palcA. As part of a functional test testing the efficiency of palcA, this construct was ran as a negative control.

Test sample:

T--TAS_Taipei--_Fig_2-3_Purified_Protein_Gel_ALDH2.JPG


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2611
    Illegal BamHI site found at 1615
    Illegal BamHI site found at 2353
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1319
    Illegal AgeI site found at 1447
    Illegal AgeI site found at 1978
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None