Composite

Part:BBa_K2549038

Designed by: Rongrong Du   Group: iGEM18_Fudan   (2018-10-09)
Revision as of 10:38, 9 October 2018 by Registry (Talk | contribs)

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CfaC-ZF21.16C-NLS

This part is one of the downstream elements of our amplifier. It is constructed by fusing CfaC, ZF21.16C and NLS, from N terminal to C terminal. CfaC is the C-terminal fragment of Cfa which is a consensus sequence from an alignment of 73 naturally occurring DnaE inteins that are predicted to have fast splicing rates. ZF21.16C is the C-terminal fragment of the zinc finger whose recognition helices for three-finger arrays are substituted by the reported synthetic zinc finger 21.16 residues on the basis of the BCR_ABL-1 artificial zinc finger. NLS is a short nuclear location sequence from SV40 large T antigen. When coexpressed with VP64-ZF21.16N-CfaN in the same cell, both fusions are formed and a transcription activating function is executed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 91
    Illegal SapI.rc site found at 21


[edit]
Categories
//proteindomain/binding
//proteindomain/internal/special
//proteindomain/localization
Parameters
None