Coding

Part:BBa_K2692000:Design

Designed by: Kristi Turton   Group: iGEM18_Lethbridge_HS   (2018-10-08)
Revision as of 19:45, 8 October 2018 by Registry (Talk | contribs)

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CutA metal binding protein from E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The his tag has the ability to also bind to metals (this is why is is able to bind to a nickle column). To gain accurate results, the his tag would have to be removed to ensure that the cutA protein is binding the copper.


Source

The part in plasmid was a gift from the Weiden lab at the University of Lethbridge and was a part of the ASKA database.

References