Coding

Part:BBa_K2668010:Design

Designed by: Younes Bouchiba   Group: iGEM18_Toulouse-INSA-UPS   (2018-08-16)
Revision as of 21:54, 9 October 2018 by Younes (Talk | contribs)


Cerberus : A Molecular Binding Plateform (mSA2-CBM3a-AzF)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 358
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part BBa_K2668010 comes from the Cerberus Project. It was design for binding cellulose to different types of compounds at the same time thanks to its two very unlike linkers. The streptavidin linker can bind biotin and biotinylated compounds while the UnAA-containing linker will click molecules that bear an alkyne function.


Source

The Part was obtained by IDT gBlock synthesis. The CBM3a domain is a corrected version of the Part:BBa_K1321014, RFC10 compatible . The monomeric streptavidin is the Part:BBa_K1896000.


References